Please wait a minute...

大连海洋大学学报  2007, Vol. 22 Issue (1): 53-57    DOI: 10.3969/j.issn.1000-9957.2007.01.012
  |
用复合酶酶解-热水浸提法提取裙带菜孢子叶粉岩藻聚糖硫酸酯的工艺研究
何云海,汪秋宽,苏唱
大连水产学院,辽宁省水产品加工及综合利用重点开放实验室,辽宁,大连,
The extracting technology of fucoidan from sporophyll powder of sea mustard （Undaria pinnatifuta） suringar by enzymatic hydrolysis
HE Yun-hai, WANG Qiu-kuan, SU Chang
Key and Open Laboratory of Fishery Product Processing and Utilization of Liaoning Province, Dalian Fisheries Univ., Dalian 116023, China
下载:  PDF (3548KB) 
输出:  BibTeX | EndNote (RIS)      
摘要 采用正交试验方法，研究了用戊聚糖复合酶及复合纤维紊酶酶解-热水浸提法提取裙带菜Undariapinnatifida孢子叶粉中岩藻聚糖硫酸酯的酶解工艺参数。结果表明：用戊聚糖复合酶酶解裙带菜孢子叶粉的最佳酶解参数为酶加量0．12％，温度为50℃，pH为5．5，时间为30min，岩藻聚糖硫酸酯粗提物得率为5．171％，总硫酸根的质量分数为1．432％，粗提物中糖的质量分数为21．38％；用复合纤维素酶酶解裙带菜孢子叶粉的最佳酶解参数为酶加量0．227％，温度为45℃，pH为4．5，时间为70min，岩藻聚糖硫酸酯粗提物得率为5．720％，总硫酸根的质量分数为1．118％，粗提物中糖的质量分数为28．24％。用两种复合酶酶解-热水浸提法提取的岩藻聚糖硫酸酯粗提物得率均较水煮法（4．665％）高。
服务
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章
何云海
汪秋宽
苏唱
关键词:  岩藻聚糖硫酸酯  复合酶解  提取工艺  裙带菜孢子叶粉    
Abstract: The parameters of the two enzymatic hydrolyzing technology with hot water were determined in extracting fucoidan from sporophyll powder of sea mustard Undaria pinnatifida. The optimum factors were determined for Novozymes Viscozyme L through the orthogonal test： the Novozymes Viscozyme L added 0. 12%, temperature 50℃, pH 5.5 and 30 minutes. The yield rate of fucoidan extracted was 5. 171% under the optimal conditions, in which the content of SO4^2- was 1. 432% , and the content of polysaccharides was 21.38%. The optimum factors for Celluclast BG were as the following： the Celluclast BG added 0. 227%, temperature 45℃, pH 4.5 and 70 minutes,the yield rate of fucoidan extracted being 5. 720%, in which the content of SO4^2- was 1.118%, and the content of polysaccharides was 28. 24%. The yield rate of fucoidan extracted was higher by enzymatic hydrolysis with hot water extracting technology than by the traditional extracting technology（4. 665% ）.
Key words:  fucoidan    combined enzymatic hydrolysis    extracting technology    sporophyll powder of Undaria pinnatifida
                    发布日期:  2016-12-30      期的出版日期:  2007-02-21
中图分类号:  TS254.5  
引用本文:    
何云海, 汪秋宽, 苏唱. 用复合酶酶解-热水浸提法提取裙带菜孢子叶粉岩藻聚糖硫酸酯的工艺研究[J]. 大连海洋大学学报, 2007, 22(1): 53-57.
HE Yun-hai, WANG Qiu-kuan, SU Chang. The extracting technology of fucoidan from sporophyll powder of sea mustard （Undaria pinnatifuta） suringar by enzymatic hydrolysis. Journal of Dalian Ocean University, 2007, 22(1): 53-57.
链接本文:  
https://xuebao.dlou.edu.cn/CN/10.3969/j.issn.1000-9957.2007.01.012  或          https://xuebao.dlou.edu.cn/CN/Y2007/V22/I1/53
[1] 李晓旭, 刘舒, 洪滨, 刘文博, 任丹丹, 汪秋宽, 何云海, 洪瑞晨, 付开艳, 宋悦凡. 裙带菜岩藻聚糖硫酸酯酸解工艺优化及降解产物对巨噬细胞的抗炎活性[J]. 大连海洋大学学报, 2022, 37(1): 166-173.
[2] 谈俊晓, 赵永强, 李来好, 杨贤庆, 吴燕燕, 魏涯, 岑剑伟. 响应面优化南极磷虾虾青素的复合酶法提取工艺研究[J]. 大连海洋大学学报, 2018, 33(4): 514-521.
[3] 张雪迪, 宋悦凡, 汪秋宽, 何云海, 刘舒, 任丹丹, 武龙, 丛海花. 苷苔岩藻聚糖硫酸酯分离纯化及结构研究[J]. 大连海洋大学学报, 2017, 32(6): 740-746.
[4] 姜龙, 宋悦凡, 罗宣, 武龙, 丛海花, 汪秋宽. 树皮藻岩藻聚糖硫酸酯的纯化及其化学结构研究[J]. 大连海洋大学学报, 2017, 32(1): 73-78.
[5] 王庆钧, 谷越, 杨颖, 汪秋宽, 宋悦凡, 何云海, 任丹丹, 丛海花. 萱藻中岩藻聚糖硫酸脂的提取工艺优化及其保肝护肝作用[J]. 大连海洋大学学报, 2015, 30(4): 417-421.
[6] 李鸶鸶, 汪秋宽, 何云海, 任丹丹, 张泽宇. 多肋藻岩藻聚糖硫酸酯的提取及其降血脂作用研究[J]. 大连海洋大学学报, 2013, 28(1): 94-98.
[7] 刘远平, 张琳琳, 张慧怡, 赵丹, 马然, 汪秋宽. 海燕体壁胶原蛋白提取工艺的初步研究[J]. 大连海洋大学学报, 2011, 26(1): 53-57.
[8] 张婷, 汪秋宽, 刘建伟, 谢智芬, 史永富, 何云海. 用弱碱性阴离子树脂分离纯化海带岩藻聚糖硫酸酯工艺的研究[J]. 大连海洋大学学报, 2008, 23(2): 153-156.
[9] 何云海, 汪秋宽, 刘红丹. 用复合酶酶解提取海带岩藻聚糖硫酸酯的工艺研究[J]. 大连海洋大学学报, 2006, 21(1): 55-58.
No Suggested Reading articles found!
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed