Abstract: Acid-soluble collagen(ASC)and pepsin-solubilized collagen(PSC)were extracted from body wall of star fish Asterina pectinifera by two different methods.The optimum extraction of ASC with the yield of 7.93%were found at hydrolyzing temperature of 10℃,hydrolyzing time of 72 h and the concentration of 0.5 mol/L acetic acid -sodium acetate.The optimum conditions for the extraction of PSC with yield of 49.61%were found as follows:hydrolyzing temperature of 17℃,hydrolyzing time of 96 h,and the pepsin dose of 4%.The UV spectra and Amino Acid Analysis revealed that ASC and PSC from body wall of the star fish were classified as type I collagen.