Abstract: Acute toxicity of “Shuangxiang” oil dispersant and dispersed oil to redfin pufferTakifugurubripeswith body weight of (27.3±0.66) g was studied at water temperature of (21.0±0.5)℃. The median lethal concentration(LC50) value of the oil dispersant was found to be decreased with time elapsed from 24 to 96 h, with a power model of LC50=9452.2t-0.9529,R2=0.8794 between LC50of oil dispersant and time (t). The relationship between LC50of dispersed oil and time (t) was described by a linear model of LC50=-0.0303t+11.244,R2=0.891. The 96 h LC50was 146.41 mg/L for oil dispersant and 8.45 mg/L for dispersed oil, indicating that the toxicity was higher in dispersed oil (high toxicity) than that in oil dispersant(general toxicity). The redfin puffer exposed to the oil dispersant and dispersed oil showed toxic symptoms including slowly swimming near the water surface, losing balance, lying on the bottom and death when congestion and enlargement in gill filaments, and more mucus on gill filaments and body surface.