Abstract: The intestinal microbial community structure and diversity were studied in silver carpHypophthalmichthysmolitrixand bighead carpAristichthysnobilisby PCR-DGGE (Polymerase chain reaction-denaturing gradient gel electrophoresis) technique. PCR-DGGE fingerprinting showed that the silver carp had an average band of 16, with few differences between individuals, and the bighead carp had 14 bands on an average, with large differences between individuals. UPGMA cluster analysis and similarity analysis of PCR-DGGE fingerprint bands revealed that the silver carp had average similarity coefficient of 0.569,at a medium similar level, and the bighead carp had average similarity coefficient of 0.623,also at a medium similar level. The sequencing results showed that the main intestinal microbials in silver carp were consisted of Bacteroidetes, Firmicutes, Proteobacteria and Verrucomicrobia, and Bacteroidetes, Proteobacteria, Cyanobacteria and Firmicutes were found in the main intestinal microbials in the bighead carp.
张金勇, 高养春, Amir Shah Ruddin Md Sah, Aileen TAN Shau-Hwai, Norlaila Binti Mohd Zanuri, 李宏俊. 基于高通量测序和形态学鉴定的马来西亚马塘红树林区浮游动物多样性分析[J]. 大连海洋大学学报, 2021, 36(6): 910-919.
2014, Vol. 29 
