Effects of low temperature stress on gut microbial community structure of different strains of obscure puffer Takifugu fasciatus
WANG Qian,ZHAI Wanying,HAN Shuang,ZHU Xinpeng,TU Hanqing,CHEN Liangbiao
1.Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai 201306,China;2.International Research Center for Marine Bioscience,Ministry of Science and Technology,Shanghai Ocean University,Shanghai 201306,China;3．Jiangsu Zhongyang Group Company Limited,Hai’an 226600,China
Abstract: In order to evaluate the diversity of fish gut microbiome under low temperature and reveal the different effects of low temperature stress on gut microbiome community structure of different strains of obscure puffer Takifugufasciatus with body mass of 300 g,gut microbiome community diversity was investigated in cold-resistant“Zhongyang No.1”and wild-type obscure puffer exposed to 4 ℃ from 26 ℃ at a continuous cooling rate of 0.5 ℃/h，and then the total genome DNA of gut microbiome of the “Zhongyang No.1”and wild-type obscure puffer was detected at 17,10,and 6 ℃ by Illumina sequencing technology.The results showed that there was similar gut microbiome in the two strains of obscure puffer,with different relative abundance.The “Zhongyang No.1”had lower OTUs,richness and diversity of gut microbiome than the wild-type obscure puffer,and tended to be consistent with the composition of gut microbiome after long-term low temperature acclimation.There was higher relative abundance of norank_f_Arcobacteraceae and Brevinema in gut microbiome of “Zhongyang No.1”than that in the wild-type obscure puffer.Function prediction indicated that “Zhongyang No.1”obscure puffer had higher abundance of function such as carbohydrate transport and metabolism,lipid transport and metabolism in the gut compared with the wild-type.It is concluded that the gut microbiome diversity was significantly lower in “Zhongyang No.1”than that in wild-type,and decline in the diversity of gut microbiome in “Zhongyang No.1”with the decrease in temperature,indicating that low temperature may have an important effect on reducing the diversity of fish gut microbiome.
汪倩, 翟万营, 韩爽, 朱新鹏, 涂翰卿, 陈良标. 低温胁迫对不同品系暗纹东方鲀肠道微生物群落结构的影响[J]. 大连海洋大学学报, 2022, 37(1): 80-87.
WANG Qian, ZHAI Wanying, HAN Shuang, ZHU Xinpeng, TU Hanqing, CHEN Liangbiao. Effects of low temperature stress on gut microbial community structure of different strains of obscure puffer Takifugu fasciatus. Journal of Dalian Ocean University, 2022, 37(1): 80-87.
2022, Vol. 37 
