Bacterial flora structures in intestinal tract and pond environment of sea cucumber under three water quality regulation methods in spring
GUO Chao, ZHANG Dong-sheng, LIN Qing, CHEN Ji-feng, WEI Ya-nan,SUN Guang-wei, LEI Zhao-lin, ZHOU Wei
College of Fisheries and Life Science, Dalian OceanUniversity, Dalian 116023,China;Innovation andEntrepreneurship College, Dalian Ocean University,Dalian 116023,China
Abstract: Bacterial flora structures in intestinal tract and pond environment were investigated in a sea cucumber Apostichopus japonicus pond of 600 m in length×85 m in width, 1.2-2.0 m in depth 3 days before spring tide in March of 2017 in coastal Dalian under three water quality regulation methods including a 750 W water quality regulator, a micropore aeration system, and natural pond without any aeration system by 16 S rRNA high throughput sequencing method. The results showed that there were 10 phyla of bacterial preponderance flora including Proteobacteria, Bacteroidetes, Verrucomicrobia, Firmicutes, Planctomycetes, Actinocteria, Cyanobacteria, Chloroflexi, Acidobacteria, and Gemmatimonadetes in the pond water, sediment and intestinal tract. The bacteroidetes(61.9%) were found to be the first dominant phylum in the ponds environment and intestinal tract with three water quality regulation methods, besides proteobacteria(>55.9%) were the first dominant phylum in the sea cucumber ponds water with micropore aeration systems. The minimal Simpon index and the maximal indices of Chao1, ACE and Shannon were observed in water and sediments in the sea cucumber ponds with water quality regulators, meanwhile the maximal ACE and Shannon indices were seen in the intestines in the sea cucumber ponds with water quality regulators. The findings indicate that water quality regulators could promote the diversity of bacteria in the pond, with the maximal diversity of bacteria in the testiness of the sea cucumber in the ponds with water quality regulators.
2019, Vol. 34 
