Effects of different algae and polyculture ratio of sea cucumber Apostichopus japonicus and sea urchin Hemicentrotus pulcherrimus on growth，body composition and digestive enzyme activities in sea cucumber and sea urchin
SHI Jiageng1,2,3, FENG Yanwei1*, JIANG Xu1, LIU Xiangquan1
1.Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Resource and Environment Research Institute, Yantai 264006, China; 2.National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; 3.Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China
Abstract: Sea urchin Hemicentrotuspulcherrimus with body weight of (0.38±0.15) g were polycultured with sea cucumber Apostichopusjaponicus with body weight of (1.24±0.17) g in a 30 cm×40 cm×50 cm white plastic tank at initial sea cucumber stocking density of 20 g and at initial sea urchin stocking density of sea urchin to sea cucumber=3∶1, 2∶1, 1∶1, 1∶2 and 1∶3 in volume, and fed macroalgae Leathesiadifformis (group H, H1-H5) or Ulvapertusa (group L, L1-L5) at water temperature of 9.5-16.5 ℃ for 40 days, and in control group (group D), only sea cucumber was reared at initial stocking density of 20 g and fed 20 g formulated feed without macroalgae to investigate effects of different algae and sea cucumber-sea urchin ratios on the growth, body composition and digestive enzyme activity in sea urchin and sea cucumber. It was found that the weight gain rate (WGR) and specific growth rate (SGR) of sea cucumber were significantly influenced by algae species and sea cucumber-sea urchin ratios (P<0.05), with the maximal SGR (0.94%/d) of sea cucumber in group H1, and the maximal SGR(1.36%/d) of sea cucumber in group L2. The growth of sea urchin was significantly affected by algae species and sea cucumber-sea urchin ratios (P<0.05), with decrease in SGR with the increase in its own stocking density and significantly higher in groups L than that in groups H at the same polyculture ratio (P<0.05). The activities of intestinal trypsin and amylase were shown to be decrease with the decrease in the ratio of sea cucumber to sea urchin, higher in groups L1 and L2 , without significant difference (P>0.05). There were higher activities of trypsin and amylase in groups L than those in groups H at the same ratio of sea cucumber to sea urchin, while the activity of lipase was only affected by the ratio of sea cucumber to sea urchin, decreased with the decrease in the ratio of sea cucumber to sea urchin. The crude protein contents were found to be increased first and then decreased with the decrease in the ratio of sea cucumber to sea urchin in groups L, with the maximal value (39.10%) in group L3, significantly higher than that in other groups (P<0.05). According to the above results, when U.pertusa was used as food, the suitable ratio of sea cucumber to sea urchin was 2∶1, while the suitable ratio of sea cucumber to sea urchin was 3∶1 when L.difformis was used as food. The findings provide references with the establishment of polyculture model.
时嘉赓, 冯艳微, 姜绪, 刘相全. 不同藻类与混养比例对刺参与马粪海胆生长、体成分和消化酶活性的影响[J]. 大连海洋大学学报, 2020, 35(4): 509-515.
SHI Jiageng, FENG Yanwei, JIANG Xu, LIU Xiangquan. Effects of different algae and polyculture ratio of sea cucumber Apostichopus japonicus and sea urchin Hemicentrotus pulcherrimus on growth，body composition and digestive enzyme activities in sea cucumber and sea urchin. Journal of Dalian Ocean University, 2020, 35(4): 509-515.
2020, Vol. 35 
