Abstract: A 90-day feeding trial was conducted to evaluate effects of dietary betaine hydrochloride (BHE) on growth performance, intestinal digestive enzyme activities and immunity of sea cucumberApostichopusjaponicus. Juvenile sea cucumber with an initial body weight of (2.32±0.04)g were reared in a 60 L white plastic tank and fed basal diet (19.83% crude protein, 4.59% crude lipid) as a control group and the basal diets containing betaine hydrochloride levels of 0.2%, 0.4%, 0.6%, and 0.8% with 3 replicates at water temperature from 10.2 ℃ to 25.8 ℃ and a salinity of 33-35. The results showed that the specific growth rate was significantly enhanced in the sea cucumber fed the diets containing 0.2%-0.6% of betaine hydrochloride (P<0.05),though no mortality was observed in the sea cucumber fed the various diets during the feeding trial. BHE supplementation led to significantly increase apparent digestibility of dry matter (P<0.05) and gutted body weight rate (GBWR)in sea cucumbers, with significant difference only in the sea cucumber fed the diets containing 0.4%-0.6% of (BHE)(P<0.05). There was significantly increase in activities of protease in intestine of sea cucumber fed diets supplemented with 0.2%-0.6% of BHE (P<0.05). On the 60th day during the feeding trail, however, significantly decrease in activities of amylase in intestine in the sea cucumber fed the diet supplemented with BHE (P<0.05). BHE supplementation resulted in increase in activities of superoxide dismutase (SOD) and alkaline phosphatase (AKP) in intestine of the sea cucumber, significantly higher in the sea cucumber fed diet with 0.4% BHE than those in the control diet (P<0.05). The findings indicate that growth, intestinal protease activities as well as immunity in the sea cucumber are improved by supplemented with BHE at a dose of 0.2%-0.6%. It is recommended that the optimal dietary betaine hydrochloride supplemental level be 0.4%.
刘佳亮, 王琳超, 曹学彬, 李静, 李君华, 宋盛亮, 王福辰, 刘石林. 饲料中添加盐酸甜菜碱对刺参幼参生长、消化酶活力和免疫力的影响[J]. 大连海洋大学学报, 2018, 33(5): 570-575.
LIU Jia-liang, WANG Lin-chao, CAO Xue-bin, LI Jing, LI Jun-hua, SONG Sheng-liang, WANG Fu-chen, LIU Shi-lin. Effectsofdietarybetainehydrochlorideongrowthperformance,intestinaldigestiveenzymeactivitiesandimmunityofjuvenileseacucumberApostichopusjaponicus. Journal of Dalian Ocean University, 2018, 33(5): 570-575.
2018, Vol. 33 
