A pathogen in mass death of Pacific white leg shrimp Litopenaeus vannamei in Liaoning Province
YAO Hong1，ZHANG Ji-peng1，YANG Chuan1，ZHANG Li-juan1，PIAO Yuan-zhi2，LIU Yue-fen2，YE Shi-gen1，LI Hua1
1.Key Laboratory of Mariculture&Stock Enhancement in North China’s Sea，Ministry of Agriculture，Dalian Ocean University，Dalian 116023，China；2.Panjin Fisheries Technique Extending Station，Panjin 124010，China
Abstract: In this paper，white spot syndrome virus（WSSV），Taurus virus（TSV），Infectious hypodermal，hematopoietic necrosis virus（IHHNV）and pathogenic bacterium Aeromonas hydrophila were identified in diseased and mass death of Pacific white leg shrimp Litopenaeus vannamei collected from Panjin region in Liaoning Province with specific primer after extracting DNA/RNA from gill tissue，16S rDNA sequencing and double PCR identification in specific primer and aerolysin gene of the pathogenic bacterium was identified to classify strain QD1.The tissue pathological observation was carried out on gills，stomach，hepatopancreas and appendages of the diseased shrimps. The results showed that WSSV and TSV were positive to the disease，and that the isolated strain was proved to be classified as Aeromonas hydrophila which led to the shrimp death by artificial challenged.Histopathological observation revealed that there was a large number of nuclei of WSSV in gill and stomach of the diseased shrimp.The severe atrophical epithelial cells，larger lumen，part of the hepatic cell necrosis and blood cells were observed in the hepatopancreas of the diseased shrimp.Comprehensive analysis confirmed that it is the mixed infection of 2 species of virus and Aeromonas hydrophila in a large number of Pacific white leg shrimp death.
姚洪, 张吉鹏, 杨川, 张利娟, 朴元植, 刘月芬, 叶仕根, 李华. 辽宁一例凡纳滨对虾大规模死亡的病原研究[J]. 大连海洋大学学报, 2016, 31(3): 256-260.
YAO Hong, ZHANG Ji-peng, YANG Chuan, ZHANG Li-juan, PIAO Yuan-zhi, LIU Yue-fen, YE Shi-gen, LI Hua. A pathogen in mass death of Pacific white leg shrimp Litopenaeus vannamei in Liaoning Province. Journal of Dalian Ocean University, 2016, 31(3): 256-260.
2016, Vol. 31 
