Isolation, virulence and ERIC-PCR genotyping of Aeromonas hydrophila in farmed turbot Scophthalmus maximus (L.)
XU Yang1,2, YAO Hong1, QIAO Guo2, LI Hua1, YE Shi-gen1, LI Rui-jun1, LI Qiang2
1.Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China；2.College of Ocean and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, China
Abstract: The isolates from naturally diseased turbot Scophthalmusmaximus (L.) in Huludao City, Liaoning Province from 2014 to 2015 were indentified and characterized by 16S rRNA gene sequence analysis and double PCR using Aeromonashydrophila-specific and aerosol gene primers. The seven isolates were shown to be A.hydrophila, with aerosol genes. The isolates genotyping by ERIC-PCR demonstrated that seven A.hydrophila strains belonged to genotype I and genotype II, in which three strains of type I were isolated from Suizhong farms, one strain of type I from Xingcheng farms and another three strains of type II were from Xingcheng farm. Moreover, the challenge tests by these seven A.hydrophila strains showed that all the strains were highly pathogenic to turbot, with the 7-days cumulative mortality(1.0×108 cells/mL) of 100%. The finding provides a theoretical basis for future clinical diagnosis and disease prevention and control of cultured turbot.
徐阳, 姚洪, 乔帼, 李华, 叶仕根, 黎睿君, 李强. 养殖大菱鲆感染嗜水气单胞菌的分离、毒力分析及ERIC- PCR分型[J]. 大连海洋大学学报, 2018, 33(4): 430-434.
XU Yang, YAO Hong, QIAO Guo, LI Hua, YE Shi-gen, LI Rui-jun, LI Qiang. Isolation, virulence and ERIC-PCR genotyping of Aeromonas hydrophila in farmed turbot Scophthalmus maximus (L.). Journal of Dalian Ocean University, 2018, 33(4): 430-434.
2018, Vol. 33 
