Abstract: An 8-week 3×2 two-factorial feeding trial was conducted to study the effects of feeding frequency(7 times/d, 5 times/d, and twice/d) and carbohydrate (sucrose and cornstarch) on growth, and activities of metabolic enzymes and digestive enzymes in Pacific white leg shrimpLitopenaeusvannameiwith initial body weight of(0.38+0.01)g. The results showed that the shrimp fed diets containing sucrose and corn starch had significantly higher final body weight and specific growth rate at feeding frequency of 7 times/d and 5 times/d than those at twice a day (P<0.05). The maximal food conversion ratio was observed in the shrimp fed twice a day, with significantly higher twice a day and 5 times/d in sucrose group than that in cornstarch group (P<0.05).The activities of malate dehydrogenase and pepsin were shown to be significantly elevated with increase in feeding frequency in the hepatopancreas of the shrimp (P<0.05), the maximal activities of kinase and lipase and significant difference(P<0.05). The interaction between feeding frequency and sugar sources showed a significant effect on the activity of glucokinase in the hepatopancreas of the shrimp with the descending order as 5 times/d>7 times/d> twice a day(P<0.05).The findings indicate that there is no significant difference in sugar sources between the two groups, and that high feeding frequency leads to high specific growth rate, and metabolic enzyme and digestive enzyme activities.
2018, Vol. 33 
