Abstract: Changes in contents of adenosine triphosphate(ATP), glycogen, and phosphate arginine in adductors, and contents of pyruvic acid and octopine and activities of the key enzymes in sugar metabolism including phosphofructokinase (PFK), opine dehydrogenases(OpDHs), and citrate synthase (CS) were monitored in fresh yesso scallop Patinopectenyessoensis stored in a refrigerator at 4 ℃ air exposure 0, 1, 2, and 3 d after storage to establish the quality evaluation index of the live scallop products. It was found that the ATP content in the adductor was decreased gradually with weakening activity of the scallop during storage, with ATP content of 3.88 μmol/g at 0 d, and almost complete exhaustion at 3 d(P<0.05). The glycogen (as main energy storage substance) content was shown to be 16.38 mg/g at 0 d, and decreased to 10.11 mg/g at 1 d, with little change due to weakened metabolism from the 2nd day on. The initial arginine phosphate(ArP) content was 3.66 μmol/g, which was almost completely consumed 3 days after storage(P<0.05), and the pyruvate and octopine were accumulated during storage process when the PFK activity was increased first and then decreased; the CS activity was very low at 0 d, and gradually decreased over time. There was the maximal activity of octopine dehydrogenase (ODH) in OpDHs, and increase during storage, i.e. 1.74 U/mg at 0 d and 2.19 U/mg at 3 d, showing that the scallop are primarily anaerobic metabolism under dry conditions. The findings indicate that ODH has the potential to be an indicator for evaluation of the quality of live scallops.
2019, Vol. 34 
