Effects of enzymatic hydrolysis products of oyster on sleeping improving
ZHANG Ting1, QIN Xiaoming1,2*, ZHANG Chaohua1,2, CAO Wenhong1,2, ZHENG Huina1,2, GAO Jialong1,2, LIN Haisheng1,2
1.College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China; 2.Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, National Research and Development Branch Center for Shellfish Processing (Zhanjiang), South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Zhanjiang 524088, China
Abstract: In order to explore the effect of enzymatic products of oysters (EPO) on sleep improving, the experiment mice were divided into negative control group (equal volume of distilled water by gavage), positive control group (diazepam 2 mg/kg body mass by gavage) and low, medium and high dose EPO groups (gavage EPO 250, 500 and 1 000 mg/kg body mass, respectively, denoted as EPO-L, EPO-M and EPO-H). After gavage once a day for 30 days, direct sleep experiment, prolonged pentobarbital sodium sleep time experiment, pentobarbital sodium subthreshold dose hypnosis experiment and incubation period experiment of barbital sodium were performed, the ELISA kit were to detect the contents of serotonin (5-HT), gamma-aminobutyric acid (GABA), melatonin (MT), norepinephrine (NE) and dopamine (DA) in mice hypothalamus and cerebral cortex. The results showed that significant prolongation of the sleep time (P<0.05), and shortening the sleep latency (P<0.05) were observed in the mice in the three doses of EPO groups compared with that in the negative control group, without direct sleep effect. There was significant increase in the sleeping rate of the mice in the medium dose of EPO group compared with that in the negative control group (P<0.05). The mice in the low and medium dose of EPO groups had significant increase in the contents of hypothalamic 5-HT and GABA (P<0.05), and significant decrease in the contents of hypothalamic DA and NE (P<0.05)compared with the mice in the negative control group did, with significant decrease in the hypothalamus DA content in the high dose group (P<0.05). The contents of GABA in cerebral cortex were significantly increased in low dose EPO group (P<0.05), the contents of GABA and MT in cerebral cortex were significantly increased, and the content of DA in cerebral cortex was significantly decreased in medium dose EPO group (P<0.05). The significant increase in MT content of cerebral cortex, and significant decrease in DA and NE contents of cerebral cortex (P<0.05) were found in the high dose group. The findings indicated that EPO led to improve sleep by increasing the contents of inhibitory neurotransmitters 5-HT, GABA and MT in the brain, by reducing the contents of excitatory neurotransmitters DA and NE, by shortening the sleep latency period, and by increasing the incidence of sleep and prolonging sleep time.
2021, Vol. 36 
