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大连海洋大学学报  2009, Vol. 24 Issue (5): 449-452    DOI: 10.3969/j.issn.1000-9957.2009.05.013
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牡蛎蛋白水解产物中α-葡萄糖苷酶活性抑制组分的分离与纯化
胡建恩,马驰宇,王刚,曲敏,谢智芬,王佳培,佟长青
大连水产学院食品工程学院 中国科学院大连化学物理研究所
Isolation and purification of α-glucosidase inhibiting components from oyster protein hydrolysate
HU Jian-en, MA Chi-yu, WANG Gang, QU Min, XIE Zhi-fen , WANG Jia-pei , TONG Chang-qing
1. School of Food Engineering, Dalian Fisheries Univ. , Dalian 116023, China;2. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
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摘要 采用硫酸铵沉淀法从大连湾牡蛎Ostrea talienwhanensis crosse软体部分中获得水溶性蛋白质,发现胃蛋白酶水解牡蛎蛋白质产物的部分组分具有α-葡萄糖苷酶(AGD)抑制活性,其AGD抑制活性IC50值为40 mg/mL。经过色谱分离纯化,并经MALDI-TOF/MS质谱检测,最终在离子交换HPLC纯化中反映出的单一吸收峰,表现出4个核质比不同的质谱峰,说明是非单一物质,因而没有进行结构分析。根据牡蛎蛋白质水解产物的AGD抑制作用,牡蛎蛋白具有开发为降血糖产品的可能性。
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胡建恩
马驰宇
王刚
曲敏
谢智芬
王佳培
佟长青
关键词:  牡蛎蛋白  酶解产物  生物活性肽  α-葡萄糖甘酶  糖尿病    
Abstract: Water - soluble proteins were produced from soft part of oyster ( Ostrea talienwhanensis crosse) by ammonium sulfate precipitation method. A part of component of the pepsin hydrolyzed oyster protein was found to have a -glucosidase inhibiting activity with IC50 value of 40 mg/mL. However, the MALDI- TOF/MS analysis revealed that there were four mass -to -charge in the ultimate chromatographic absorption peaks on ion -exchange HPLC. The structure of the pepsin hydrolyzed oyster protein was not analyzed because the pepsin hydrolyzed oyster protein was not a single compound. The α - glucosidase inhibiting activity of the oyster protein hydrolysate showed the feasibility of empoldering hypoglycemic product.
Key words:  oyster protein    hydrolysate    bioactive peptide    α- glucosidase    diabetes
                    发布日期:  2016-12-30      期的出版日期:  2009-10-21
中图分类号:  S986.2%Q516  
引用本文:    
胡建恩, 马驰宇, 王刚, 曲敏, 谢智芬, 王佳培, 佟长青. 牡蛎蛋白水解产物中α-葡萄糖苷酶活性抑制组分的分离与纯化[J]. 大连海洋大学学报, 2009, 24(5): 449-452.
HU Jian-en, MA Chi-yu, WANG Gang, QU Min, XIE Zhi-fen , WANG Jia-pei , TONG Chang-qing. Isolation and purification of α-glucosidase inhibiting components from oyster protein hydrolysate. Journal of Dalian Ocean University, 2009, 24(5): 449-452.
链接本文:  
https://xuebao.dlou.edu.cn/CN/10.3969/j.issn.1000-9957.2009.05.013  或          https://xuebao.dlou.edu.cn/CN/Y2009/V24/I5/449
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