YANG Fa-ming1, LIN Hai-sheng1,2, QIN Xiao-ming1,2*, ZHANG Chao-hua1,2, CAO Wen-hong1,2, GAO Jia-long1,2
1.College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China; 2.Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, National Research and Development Branch Center for Shellfish Processing (Zhanjiang), South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Zhanjiang 524088, China
Abstract: Trace radial diffusion method and half dilution method were used to investigate in vitro the antibacterial effects of enzymolysis products of oyster (EPO)prepared from Hong Kong oyster Crassostreasikamea by crushing and beating, and enzymatic hydrolysis of animal protease at a dose of 1000 U/g protein for 5 hours, and effects of the EPO on bleeding time and blood loss of mouse tail wound. In the open wound healing test, the skin soft tissue of SPF male mouse with body weight of (20±2)g cut off about 0.8 cm in diameter from the dorsal skin were smeared by 30-35 mg of EPO containing peptide of 1.5-2.5 mg (drug-administered group) daily, by 2-3 mg of Yunnan Baiyao daily (positive control) and without any smearing (negative control) to investigate the effects of EPO on the open soft tissue would healing. The results showed that EPO did not lead to promote procoagulant effect and inhibitory effect on 13 test common gram and positive bacteria. The animals in each test group had crusted on the 2nd day. On the 6th, 10th and 14th day, however, significantly higher wound healing rate was observed in the drug-administered group than that in the negative control group (P<0.05). Biochemical indicators showed that EPO had no significant effect on the production of inflammatory factor interleukin 6(IL-6) (P>0.05), with promoting interleukin 10(IL-10) secretion (P<0.05) on the third day. Compared with the two control groups, there was significantly lower fibroblast growth factor 2(FGF-2) content in drug-administered group than that in the two control groups (P<0.05). The transforming growth factor-β(TGF-β) and cyclin D1(CCND1)contents were shown to be significantly higher in the drug-administered group than that in the negative control groups(P<0.05), without significant difference in the epidermal growth factor(EGF) content in the drug-administered group (P>0.05). On the 7th day after administration, there was lower hydroxyproline content in the drug-administered group than that in negative control(P>0.05), elevated hydroxyprolin content in the negative control, without significant difference between the two groups (P<0.05) on the 14th day. The findings indicate that EPO functions as acceleration of wound healing in soft tissue of mouse and has a certain inhibitory effect on superficial scar hyperplasia of a mice.