Epidemiological investigation of three major viruses and Enterocytozoon hepatopenaei(EHP)in Pacific white leg shrimp Litopenaeus vannamei in Liaoning Province
WANG Bo-ya,WANG Li,LIU Mei-ru,YE Shi-gen,LI Rui-jun,LI Hua,LI Qiang
Key Laboratory of Mariculture&Stock Enhancement in North China's Sea,Ministry of Agriculture,Dalian Ocean University,Dalian 116023,China
Abstract: White spot syndrome virus(WSSV),infectious hypodermal and hematopoietic necrosis virus(IHHNV) and Taura syndrome virus(TSV)were detected by nested-PCR and RT-PCR and Enterocytozoon hepatopenaei (EHP)was detected by LAMP(Loop-mediated Isothermal Amplication)kit in diseased Pacific white leg shrimp Litopenaeus vannamei collected from 26 shrimp culture ponds in Yingkou and Panjin,Liaoning Province in August 2015.The results showed that the IHHNV had incidences of 65.4%with positive rates of 19.2%and EHP had incidences of 34.6%.While the TSV showed positive rates of 7.7%.EHP positive was found in all the small shrimp samples and seven diseased shrimp samples showed co-infection of IHHNV and EHP.There was higher positive rate in large-sized shrimp samples(100%)than in small-sized shrimp samples(0%).The findings indicated that IHHNV and EHP were major pathogens in Pacific white leg shrimp in Liaoning Province in August 2015.
王博雅, 王力, 刘美如, 叶仕根, 黎睿君, 李华, 李强. 凡纳滨对虾3种主要病毒和虾肝肠胞虫在辽宁地区的流行情况分析[J]. 大连海洋大学学报, 2017, 32(2): 150-154.
WANG Bo-ya, WANG Li, LIU Mei-ru, YE Shi-gen, LI Rui-jun, LI Hua, LI Qiang. Epidemiological investigation of three major viruses and Enterocytozoon hepatopenaei(EHP)in Pacific white leg shrimp Litopenaeus vannamei in Liaoning Province. Journal of Dalian Ocean University, 2017, 32(2): 150-154.
2017, Vol. 32 
