Pathogenicity of Vibrio harveyi and white spot syndrome virus to Pacific white leg shrimp Litopenaeus vannamei at different temperature
XIANG Yun 1, WU Yu-xue 1, WANG Gang 1, CHEN Zhao-ming 1, ZHANG Rui 2, SUN Cheng-bo 1,3
1.College of Fisheries, Guangdong Ocean University, Zhanjiang 524025, China; 2.Education Example Center, Guangdong Ocean University, Zhanjiang 524025, China；3.Tropical Marine Invertebrates Aquaculture Research Center of Guangdong Colleges and Universities, Zhanjiang 524025, China
Abstract: The pathogenicity of different concentrations of Vibrio harveyi and white spot syndrome virus (WSSV) to Pacific white leg shrimp Litopenaeus vannamei with body length of (8.12±0.83) cm was studied at water temperature of (19±1), (25±1), and (31±1)℃. Throughout the experiment, the cumulative mortality was found below 8.9% in each group and the virus carrying amount was less than 3.9×10`4 copy/g in each group at (19±1)℃. At (25±1)℃, however, the maximal cumulative mortality was 71.1%, and the virus carrying amount 1.9×10`5copy/g in the singly-infected with WSSV group, from the mid-experimental, significantly higher in the high concentrations of secondary infection group (the maximum 77.8%, 3.2×10`6 copy/g) than in the other groups (P<0.05), with higher mortality (the maximum 21.1%) and virus carrying. The maximal cumulative mortality of 47.8%，and the virus carrying amount of 2.2×10`4 copy/g were found in the singly-infected with WSSV group at (31±1)℃, significantly lower than in the secondary infection group (the maximum 80.0%，6.1×10`6 copy/g). There was increase in mortality (the maximum 24.4%) with elevated concentration of Vibrio harveyi in the singly-infected with Vibrio harveyi group. The findings indicate that the pathogenicity of Vibrio harveyi and WSSV is significantly affected by temperature, and that the pathogenicity of the secondary infections and the singly-infected with Vibrio harveyi were found to be increased with raising temperature. The pathogenicity of WSSV was shown to be inhibited by high and low temperature.
向赟, 吴宇学, 王刚, 陈兆明, 张锐, 孙成波. 不同温度下哈维氏弧菌和白斑综合症病毒对凡纳滨对虾的致病性[J]. 大连海洋大学学报, 2015, 30(1): 56-61.
XIANG Yun , WU Yu-xue , WANG Gang , CHEN Zhao-ming , ZHANG Rui , SUN Cheng-bo , . Pathogenicity of Vibrio harveyi and white spot syndrome virus to Pacific white leg shrimp Litopenaeus vannamei at different temperature. Journal of Dalian Ocean University, 2015, 30(1): 56-61.
2015, Vol. 30 
