LI Xu-wei1,2，WANG Hai-ying1，WU Rui-quan1，XIE Jun1，YU Er-meng1，WU Yin2，YU De-guang1，WANG Guang-jun1， GONG Wang-bao1
1.Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture，Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China; 2.College of Fisheries and Life Science, Dalian Ocean University,Dalian 116023, China
Abstract: Calreticulin (CRT) as a highly conserved molecular chaperone protein extensively exists in eukaryotic cells, and CRT on the surface of the cell can protect cells in temperature, and oxygen stresses, pathogens and parasitic infections.The full length cDNA of calreticulin was cloned from grass carpCtenopharyngodonidellusby race and pcr methods to ecvaluate the expression in different tissues .The gene was founf to consist of 1 389 bp with an open reading frame of 1 263 bp, encoding 421 amino acids. The amino acid sequence analysis showed that the grass carp Calreticulin had higher homology with zebra fish (86.26%), and rainbow trout (76.78%), and had 69.67% amino acid identity to murine and 69.19% to human CRT’s. Tissue expression studies showed that CRT gene was all detected but expression was different in the tested tissues including muscle, skin, intestine, hepatopancreas, kidney, spleen, gills and rays, but the expression in the hepatopancreas liver and fins was found maximum, significantly higher than in the other five tissues except in skin(P<0.05 ).
2012, Vol. 27 
