Abstract: Four monoclonal antibodies（MAbs）（1C7,1H7,2E8,and 4E9） against coelomocytes of sea cucumber Apostichopus japonicus were prepared by immunising BALB/c mice,and the characteristics were studied by the indirect immunofluorescence assay test（IIFAT）,indirect enzyme-linked immunosorbent assay（ELISA） and western-blotting.The MAb 1C7 and 1H7 were showed to have specificity for lymphoid cells,and MAb 4E9 for spherulocytes.The band MAb 2E8 was bound to all of coelomocytes.The MAb 2E8 and 4E9 were showed to be reacted with coelomic fluid components of the sea cucumber,and to be negative to MAb 1C7 and 1H7.The MAb 1C7 and 4E9 were IgG1 type,2E8 IgG2a type,and 1H7 IgM type.The western-blotting showed that MAb 1C7 and 1H7 recognised an antigen of molecular weight 37 000 protein.The MAb 4E9 108 000 protein,and MAb 2E8 reacted antigens 37 000 and 61 000 protein.This study is the preliminary step,and we will use the MAbs in study of the relationship between coelomocytes and coelomic fluid,and type,distribution,ontogenesis,differentiation and function of coelomocytes in the future.
李强, 迟刚, 李莹, 李华. 仿刺参体腔细胞单克隆抗体的制备及特性分析[J]. 大连海洋大学学报, 2010, 25(6): 542-546.
LI Qiang, CHI Gang, LI Ying, LI Hua. Preparation and characterization of monoclonal antibodies against coelomocytes of sea cucumber Apostichopus japonicus. Journal of Dalian Ocean University, 2010, 25(6): 542-546.
2010, Vol. 25 
