Comparative study on number of coelomocytes and immune enzyme activities in coelomic fluid of wild and cultured sea cucumber with different sizes
LIANG Chun-lei, REN Yuan, WANG Yi-nan, LI Qiang, YANG Huan, LI Zhen
College of Fisheries and Life Science, Key Laboratory of Mariculture & Stock Enhancement in North China s Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China;College of Marine and Biology Engineering, Yancheng Institute of Technology, Yancheng 224051, China;Dalian SEM Bio-Engineering Technology Company Postdoctoral Research Center, Dalian 116620,China
Abstract: In this trial, density, number of coelomocytes and immune enzyme activities were comparatively determined in cultured sea cucumber with body weight of(30.00±9.83)g,(70.00±7.83)g, and(110.00±10.68)g,and wild sea cucumber Apostichopus japonicus with body weight of(70.00±8.13)g,(110.00±7.53)g, and(150.00±10.64)g to explore the immunity relationship between wild and cultured sea cucumber and living environment. The results showed that there was no significant difference in the density of coelomocytes between wild and cultured animals with the different sizes(P>0.05). However, there were significantly higher density of spherulocytes in the wild sea cucumber with body weight of 150 g than that in the wild sea cucumber with body weight of 70 g and 110 g groups(P<0.05). The cultured and wild sea cucumber with the same sizes had no significantly different density of coelomocytes(P>0.05), with significantly higher density of spherulocytes and lymphoid cells in cultured sea cucumber in 110 g group than that in the wild animals(P<0.05). The differences in coelomic fluid parameters were not consistent and no certain regularity was observed in wild and cultured animals, without significant differences in these main immune enzyme activities between cultured and wild sea cucumber with the same size(P>0.05). The findings indicated that the number of coelomocytes and immune enzyme activities of sea cucumber were not influenced by environment, which provided a basis for research of the immune mechanism of sea cucumber.
梁春蕾, 任媛, 王轶南, 李强, 杨欢, 李振. 不同规格野生和养殖刺参体腔细胞数量与体腔液免疫酶活性的比较研究[J]. 大连海洋大学学报, 2019, 34(3): 316-322.
LIANG Chun-lei, REN Yuan, WANG Yi-nan, LI Qiang, YANG Huan, LI Zhen. Comparative study on number of coelomocytes and immune enzyme activities in coelomic fluid of wild and cultured sea cucumber with different sizes. Journal of Dalian Ocean University, 2019, 34(3): 316-322.
2019, Vol. 34 
