1.Chinese Academy of Inspection and Quarantine,Beijing 100029,China;2.Shekou Entry-Exit Inspection and Quarantine Bureau in Shenzhen, Shenzhen 518067,China;3.Beijing Entry-Exit Inspection and Quarantine Bureau,Beijing 100026,China
Abstract: One monoclonal antibody(MAb)(3A4)against Bohle virus(BIV)was prepared by immunizing BALB/cmice and the MAb(3A4)characteristics and application were studied by indirectenzyme-linked immunoassay(ELISA)and western-blotting.An antigen-capture ELISA was developed and the sampleswere detected by BIV.The indirect ELISA revealed that the titer ofMAb(3A4)asciteswas105,and belonged to IgG3 subclass and κchain.The MAb(3A4)did not reactwith other fish viruses or fish cells lines in the ELISA test except BIV,Andrias davidianus iridovirus(ADIV)and epizootic haematopoietic necrosis virus(EHNV).In the western blotting using MAb 3A4,a 50 000 protein band was observed when BIV was used as antigen.The antigen-capture ELISA confirmed that the ELISA was able to detect virus from amphibian,and the obtained MAb was applied to rapid diagnosis and detection of BIV in aquaculture.
2015, Vol. 30 
