Abstract: Six hybridomas (5D11,1B8,1G7,3G8,5A9 and 3H8) were prepared from Balb/C mice immunized intraperitoneally withEdwardsiellaictaluriA86(E.ictaluriA86),a pathogen of red-head disease in yellow catfishPelteobagrusfulvidraco, to secrete mAb againstE.ictaluriA86, with valence of 1∶1280 in 5D11, 1∶1280 in 1B8,1∶2560 in 1G7,1∶320 in 3G8,1∶160 in 5A9 and 1∶80 in 3H8. Bacterial cross-reaction showed that these mAbs specifically recognizedE.ictaluriA86, but showed no cross reactivity to other referred bacteria. It is worth noting that mAbs 5D11 and 1B8 were found to react withE.ictalurireferred strains. Immunofluorescence and immunohistochemistry test of the infection rule ofE.ictaluristrains in yellow catfish showed thatE.ictaluriwas detected in liver, kidney, spleen, heart, intestines, and stomach as early as 6 h after infection, the maximum in spleen, and the minimum in heart; positive signal was found in brain 24 h later, and positive signal was found in gill 30 h after infection. With the time elapse, a small amount of positive bacteria was detected around the edges of eyes 54 h after infection.
2014, Vol. 29 
