The isozyme genetic structures in large icefish (Protosalanx hyalocranius) and Taihu Lake icefish (Neosalanx taihuensis)
ZHANG Ying1, DONG Shi1, WANG Qian2, SUN Zhen-rong2
1.Department of Chemistry and Biology, Tianjin Normal University,Tianjin 300074, China; 2. Faculty of Fisheries Science, Tianjin Agricultural College, Tianjin 300384, China
Abstract: Genetic structures and genetic markers of aspartic acid transaminase (AAT), glyceraldehydes phosphate dehydrogenase(α-GPD), glucose-1-phosphate isomerase(GPI), isocitrate dehydrogenase(IDH), lactate dehydrogenase(LDH), malate dehydrogenase(MDH), phosphoglucomutase(PGM) and myogen (PROT) were examined in five populations in large icefish Protosalanx hyalocranius and one population in Taihu Lake icefish Neosalanx taihuensis by starch gel electrophoresis. The results showed that 16 loci were observed both in large icefish and Taihu Lake icefish, respectively. The proportion of polymorphic loci were 0-12.5% in large icefish and 18.8% in Taihu Lake icefish on the basis of 0.99 criterion of polymorphism; the proportion of polymorphic loci were 0 and 12.5% in two species on the basis of 0.95 criterion of polymorphism. Expected heterozygosity were 0-0.0057 and 0.0178 in two species. Five totally different loci:α-GPD-4 *, LDH-1 *, LDH-2 *, LDH-3 and PGM * were found between large icefish and Taihu Lake icefish. The Nei'genetic distances were 0.000 010-0.000 103 in large icefish, and 0.2996-0.3019 between two species, indicating that the genetic diversities were relatively lower in large icefish and Taihu Lake icefish.
张颖, 董仕, 王茜, 孙振荣. 大银鱼和太湖新银鱼同工酶遗传组成的研究[J]. 大连海洋大学学报, 2005, 20(2): 111-115.
ZHANG Ying, DONG Shi, WANG Qian, SUN Zhen-rong. The isozyme genetic structures in large icefish (Protosalanx hyalocranius) and Taihu Lake icefish (Neosalanx taihuensis). Journal of Dalian Ocean University, 2005, 20(2): 111-115.