Flora diversity in sea cucumber Apostichopus japonicus culture ponds in autumn and winter DOU Yan1, DING Jun1, QU Ling-yun2, LIU Zhi-min1
DOU Yan1, DING Jun1, QU Ling-yun2, LIU Zhi-min1,WANG Yi-nan1， MU Xiao-hu1, CHANG Ya-qing1
1.Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China; 2.First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
Abstract: Flora diversity was studied in seawater and sediments in sea cucumber Apostichopus japonicus culture ponds from Yellow Sea and Bohai Sea in autumn and winter based on total DNA extract. The 16S rDNA genes of bacteria were amplified and 16S rDNA gene libraries were constructed. The results showed that 11 known Phyla of bacteria were found in seawater and sediments: Proteobacteria, Acidobacteria, Actinobacteria, Bacteroidetes, Verrucomicrobia, Firmicutes, Fusobacteria, Cyanobacteria, Planctomycetes, Deferribacteres, and Chloroflexi, with dominant Proteobacteria(>48%). The bacterial diversity in seawater and sediments in sea cucumber culture ponds was evaluated by Simpson index and Shannon index, with the minimum (0.014 89 in Yellow Sea and 0.016 50 in Bohai Sea) in winter by Simpson index, and the maximum (6.312 in Yellow Sea and 5.695 in Bohai Sea) by Shannon index. The findings indicated that there was the maximal bacterial diversity in sediments of sea cucumber culture ponds from Yellow Sea libraries and Bohai Sea libraries in winter.
2015, Vol. 30 
