Effect of dietary chitosan oligosaccharide on bacterial community in intestinal wall of sea cucumber Apostichopus japonicus
SI Bin1,WANG Yi-nan1,LIU Hai-ying2,CAO Hai-long3,ZHANG Jian-qiang4, Lü Hui-qian1,SUN Meng-lei1,JIANG Zhi-qiang1
1.Key Laboratory of Mariculture&Stock Enhancement in North China's Sea,Ministry of Agriculture,Dalian Ocean University,Dalian 116023,China;2.College of Marine Science and Environment,Dalian Ocean University,Dalian 116023,China;3.Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China;4.Dalian Fisheries Industry Technology Innovation Association,Dalian 116023,China
Abstract: The bacterial community in intestinal wall was investigated in sea cucumber Apostichopus japonicus(5.03 g±0.03 g)fed diets containing chitosan oligosaccharide(COS)at dose of 0(G0,as control group),0.25%(G1), 0.50%(G2),0.75%(G3)and 1.00%(G4)for 50 days by high-through sequencing technique in order to evaluate the effect of dietary COS on the bacterial community structures.The results showed that there were higher bacterial diversity and abundance in the intestinal wall of the sea cucumber fed the diets containing COS than the sea cucumber fed the control diet,with dominant Proteobacteria and Firmicutes,indicating that composition of intestinal bacteria was affected by COS.The dominant genus Shewanella was observed in the intestinal wall of the sea cucumber in the control group,while the proportion of Shewanella was found to be reduced in the intestinal wall of the sea cucumber fed the diets containing COS.It was found that Bacillus was increased in amount even became the dominant bacteria in the intestinal wall of the sea cucumber fed the diets containing COS compared with the sea cucumber fed the control diet.In group G4,however,there was lower relative proportion of various bacterial genera, high dose of COS showing inhibitory effect of bacterial diversity.The findings suggest that only optimal dose of dietary COS improve bacterial diversity and abundance in the intestinal wall of the sea cucumber.
司滨, 王轶南, 刘海映, 曹海龙, 张建强, 吕绘倩, 孙梦蕾, 姜志强. 饲料中添加壳寡糖对刺参肠壁菌群的影响[J]. 大连海洋大学学报, 2017, 32(2): 167-171.
SI Bin, WANG Yi-nan, LIU Hai-ying, CAO Hai-long, ZHANG Jian-qiang, Lü Hui-qian, SUN Meng-lei, JIANG Zhi-qiang. Effect of dietary chitosan oligosaccharide on bacterial community in intestinal wall of sea cucumber Apostichopus japonicus. Journal of Dalian Ocean University, 2017, 32(2): 167-171.
2017, Vol. 32 
