1.College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, China；2.Key Laboratory of Sustainable Exploitation of Oceanic Fisheries Resources, Ministry of Education, Shanghai 201306, China； 3.National Distant-water Fisheries Engineering Research Center, Shanghai 201306, China； 4.Collaborative Innovation Center for Distant-water Fisheries, Shanghai 201306, China
Abstract: Microbial diversities on skins were investigated in total genomic DNA extracted from two species of Antarctic fish Trematomusbernacchii(TB) and Nototheniacoriiceps(NC) collected from open waters in Zhongshan Station, Antarctica, China, using 16S rDNA and through high-throughput sequencing techniques and by bioinformatics analysis to evaluate the skin microbial diversities of Antarctic fishes and unfold the differences in the Antarctic fish skin microbial communities. A total of 378 517 effective sequences and 740 OTUs (Operational Taxonomic Units, OTUs) were obtained from three individuals from the Antarctic fishes. The dominant bacterial communities in the two Antarctic fishes were listed as Firmicutes, Proteobacteria, Deinococcus-Thermus, Bacteroidetes, Tenericutes and Actinobacteria at phylum level, with higher abundance of Firmicutes, Proteobacteria and Bacteroidetes. At the genus level, the dominant bacteria communities were Anoxybacillus, Vulcaniibacterium, Bacillus, Psychrobacter, Ralstonia, Brevundimonas, Pseudomonas and Methylophilus. Distance algorithm analyses revealed that there was little significant difference between individuals of the same Antarctic fish species, without significant difference between the two Antarctic fish species (P>0.05). In conclusion, the microbial diversity between two Antarctic fish was compared by using high-throughput sequencing techniques. Firmicutes, Proteobacteria and Bacteroidetes were the dominant bacterila communities in the skins, without significant difference, in the two Antarctic fish species.
2019, Vol. 34 
