Effects of probiotics on growth,digestive enzyme activity,and immune response of juvenile sea cucumber Apostichopus japonicus in a biofloc culture system
ZHANG Cong-yao,REN Meng,ZUO Ran-tao,SUN Pi-hai, YANG Zhi-ping,QIAO Yan-bing,MA Yue-xin
1.Key Laboratory of Mariculture and Stock Enhancement in North China's Sea,Ministry of Agriculture,Dalian Ocean University,Dalian 116023,China;2.Seafood Seedling Breeding Base,Dalian Ocean University,Dalian 116023,China;3 Dalian Huixin Titanium Equipment Development Company Limited,Dalian 116039,China
Abstract: Sea cucumber Apostichopus japonicus with body weight of(10.77 g±0.07 g)were randomly divided into six 20 L sand filtered sea water tanks at a density of 30 individuals per tank,and fed the basal diet(70%basal diet)containing sucrose(control).In the probiotics treatment,however,the sea cucumber was fed the same diet as in the control in the water which was added into a mixture of Bacillus subtilis(1.8 g)plus nitrifiers(1.8 g)in a 3 day interval at feeding rate of about 4%for 60 days to evaluate the effects of commercial probiotics on growth,digestion,and immune response of juvenile sea cucumber in biofloc culture system.Results showed that there was significantly higher specific growth rate in the sea cucumber fed the diet containing probiotics than that in the control during 30 d and 60 d rearing(P＜0.05).The sea cucumber in the probiotics had significantly higher intestinal amylase and lipase activities than those in control did(P＜0.05),without significant differences in trypsin activity between probiotics treatment and the control(P＞0.05).The phagocytic activity of coelomocytes and the lysozyme activity in coelomic fluid and in coelomocyte lysate supernatant were shown to be significantly enhanced in probiotics treatment compared with the control(P＜0.05).The findings revealed that probiotics supplementation improved the growth,digestion and immune response of juvenile sea cucumber in a biofloc culture system.
2017, Vol. 32 
