Abstract: A feeding trial was conducted to evaluate effects of probiotics on immune response and antioxidant performance in juvenile sea cucumber Apostichopus japonicus.The juvenile sea cucumber with body weight of（0.54± 0.06）g were fed basal diet or diet supplemented with probiotics containing Rhodotorula sp.H26（1×105cells/g diet），Metschnikowia sp.C14（1×105cells/g diet）and Bacillus sp.BC26（1×107cells/g diet）for 4 and 8 weeks. Results showed that there were significantly higher phagocytic activity and respiratory burst in coelomocytes and significantly higher lysozyme and phenoloxidase activities in coelomic fluid（CF）and coelomocytes cells lysate supernatant（CLS）in the sea cucumber fed the diet containing probiotics than those in the control（P＜0.05）in 4 weeks. All immune parameters mentioned above were siginificantly enhanced by probiotcs administration compared to the animals in the control（P＜0.05）in 8 weeks except for phenoloxidase activity.Superoxide dismutase（SOD）acivity in CF，intestine and body wall，catalase（CAT）acivity and total antioxidant capacity（T-AOC）in all tissues and glutathione（GSH）content in intestine and respiratory tree in sea cucumber fed the diet supplemented with probiotics were shown to be significantly higher than those in the animals fed basal diet（P＜0.05）in 4 weeks.SOD activity in intestine，respiratory tree and body wall，CAT activity in all tissues，T-AOC in CLS and intestine and GSH content in CF，intestine and respiratory tree were significantly elevated in the sea cucumber fed the diet supplemented with probiotcs compared to those in the control（P＜0.05）in 8 weeks.The findings revealed that diet supplemented with probiotics improved immune response and antioxidant performance in sea cucumber juveniles.
李璐瑶, 孙丕海, 包鹏云, 张丛尧, 杨志平, 马悦欣. 饲料中添加混合益生菌对刺参幼参免疫反应和抗氧化性能的影响[J]. 大连海洋大学学报, 2016, 31(3): 266-271.
LI Lu-yao, SUN Pi-hai, BAO Peng-yun, ZHANG Cong-yao, YANG Zhi-ping, MA Yue-xin. Effects of dietary supplementation of probiotcs on immune response and antioxidant performance in juvenile sea cucumber Apostichopus japonicus. Journal of Dalian Ocean University, 2016, 31(3): 266-271.
2016, Vol. 31 
