Effects of dietary chitosan on hematological parameters and non-specific immunity of redfin puffer Takifugu rubripes
SU Peng，PAN Jin-lu，HAN Yu-zhe，LIU Hai-ying，HUO Pu-yu，SUN Hang，JIANG Zhi-qiang
College of Fisheries and Life Science，Key Laboratory of Mariculture&Stock Enhancement in North China's Sea，Ministry of Agriculture，Dalian Ocean University，Dalian 116023，China
Abstract: Juvenile redfin puffer Takifugu rubripes with body weight of（129.2±3.1）g were reared in a plastic 2 m3tank at a density of 50 fish per treatment and fed a basal diet supplemented with chitosan-oligosaccharides at dose of 0（C0），500（C1），1000（C2）and 2000（C3）mg/kg for 56 days at water temperature of（17.0±2.0）℃ with triplication to evaluate the effects of chito-oligosaccharides on serum，blood biochemical parameters and non-specific immunity function of redfin puffer.The results showed that there were significantly higher contents of blood platelet and higher activities of serum alkaline phosphatase，lysozyme，catalase，and superoxide dismutase in the fish fed the diets supplemented with chitosan-oligosaccharides than those in the fish fed the control diet（P＜0.05），increase with elevated dose of chitosan-oligosaccharides.The redfin puffer juveniles fed the diets containing chitosanoligosaccharides had significantly higher activities of hepatic catalase，and superoxide dismutase and significantly lower malondialdehyde content in liver than the fish fed the control diet（P＜0.05）.The redfin puffer juveniles fed the diets containing chitosan-oligosaccharides showed high resistance to Edwardsiella tarda and Vibrio harvey.It can be concluded that dietary supplementation of chito-oligosaccharides can improve the non-specific immunity function of redfin puffer，and that the supplemental level of chitosan-oligosaccharides in diets is suggested to be 2000 mg/kg diet.
苏鹏, 潘金露, 韩雨哲, 刘海映, 霍圃宇, 孙航, 姜志强. 壳寡糖对红鳍东方鲀血液指标和非特异性免疫指标的影响[J]. 大连海洋大学学报, 2016, 31(1): 37-43.
SU Peng, PAN Jin-lu, HAN Yu-zhe, LIU Hai-ying, HUO Pu-yu, SUN Hang, JIANG Zhi-qiang. Effects of dietary chitosan on hematological parameters and non-specific immunity of redfin puffer Takifugu rubripes. Journal of Dalian Ocean University, 2016, 31(1): 37-43.
2016, Vol. 31 
