Cloning and response to pathogen stimulation of C-type lectin gene CTL5 in swimming crab Portunus trituberculatus
YAN Deping1,3, Lü Jianjian2,3, TI Xingbin3，ZHANG Wen1,3, ZHANG Yunbin3, SONG Liu3, LIU Ping2,3*
1.College of Fisheries and Life Science, Dalian Ocean University, Dalian; 2.Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao; 3.Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao
Abstract: The C-type lectin cDNA of swimming crab Portunustrituberculatus(designated PtCTL5)was cloned by rapid amplification of cDNA ends to clarify the structure and function of PtCTL5 gene.It was found that PtCTL5 as galactose-binding lectin was 1026 bp in length, containing an open reading frame of 732 bp and encoding a mature protein of 243 amino acids, with a relative molecular mass of 26 000.Blast analysis revealed that amino acid sequence of PtCTL5 had the maximal similarity with red swamp crayfish Procambarusclarkii, up to 41.84%.Phylogenetic tree analysis showed that PtCTL5 was far from being related to four other C-type lectins found from swimming crab Portunustrituberculatus.The expression of PtCTL5 gene was shown to be varied with different developmental stages, the maximum in zoea Ⅲ stage of larval development and the minimal in heart beating stage of embryo development.The PtCTL5 gene was expressed in all tested tissues with the maximum in hemocytes, followed by hepatopancreas.Under normal circumstances, after artificial infection with Vibrioparahaemolyticus, the maximal expression of PtCTL5 gene in hemocytes and in hepatopancreas at 72 h; while, exposed to WSSV infection, the peak PtCTL5 gene expression was observed hemocytes and hepatopancreas at 72 h and 12 h, respectively, significantly higher than that in the control group(P<0.05).The findings indicate that PtCTL5 plays an important role in the immune response in swimming crab and lays a reference for further research on the swimming crab immune system.
2020, Vol. 35 
