Classification of surface antigens on coelomocytes and immune cells in body wall of sea cucumber Apostichopus japonicus
ZHAO Xiao-ran;LI Yan;WANG Li-fen;CHANG Ya-qing;WANG Bin
Key Laboratory of Mariculture & Stock Enhancement in North China s Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China;Department of Pathology, the Second Affiliated Hospital of Dalian Medical University, Dalian 116027, China
Abstract: CD analog types(CD3, CD4, CD7, CD8, CD11 b, CD14, and CD38) on surface antigens on coelomocytes were investigated by flow cytometry(FCM) and localization was immunohistochemistrically carried out on immune cells in body walls in sea cucumber Apostichopus japonicus with body weight of(160±10)g in order to detect the classification of surface antigens on coelomocytes and localization of immune cells in body walls. It was found that CD7 analogues and CD14 analogues on coelomocytes with diameter of > and < 5 μm were concerned as positive surface antigens, and the positive cells CD7 and CD14 stained brown distributed in the connective tissue were observed. This finding indicated that CD7 and CD14 analogs were explored in coelomocytes and somatic tissue cells, and had immunological functions of T cells and NK cells, which provides theoretical supports for immune evolution among organisms.
赵小然, 李艳, 王莉芬, 常亚青, 王斌. 刺参体腔细胞表面抗原分类及体壁组织中免疫细胞的研究[J]. 大连海洋大学学报, 2019, 34(4): 470-474.
ZHAO Xiao-ran, LI Yan, WANG Li-fen, CHANG Ya-qing, WANG Bin. Classification of surface antigens on coelomocytes and immune cells in body wall of sea cucumber Apostichopus japonicus. Journal of Dalian Ocean University, 2019, 34(4): 470-474.
2019, Vol. 34 
