Effects of low temperature stress on expression of MT, AQP1, and TCP1 genes in low temperature-resistant and non-low temperature- resistant strains of large yellow croaker Larimichthys crocea
LI Weiye1, LUO Haizhong2, YIN Xiaolong1, ZHANG Chuan1, LIU Minhai3, ZHANG Xia1, QIU Haojun1, MAO Zhizeng1,YOU Jiuju1, XU Zhijin1*
1.Fisheries Research Institute of Zhoushan, Zhoushan 316000, China; 2.Ocean and Fishery Bureau of Zhoushan, Zhoushan 316000, China; 3.Zhejiang Wanli University， Ningbo 315100, China
Abstract: Juvenile F5 low temperature-resistant strains of large yellow croaker Larimichthyscrocea with body mass of (55.35±3.52)g and non-low temperature resistant strains of large yellow croaker with body mass of (56.45±4.41)g were reared in a round fiberglass tank with 200 cm diameter and 100 cm height at stocking density of 50 fish per tank at initial water temperature of 16 ℃,and cooling by 1 ℃ daily, and then expressions of MT, AQP1, and TCP1 genes were investigated in liver and muscle of large yellow croaker at water temperature of 16, 13, 10, and 7 ℃ after the low temperature stress by the Quantitative Real-time PCR in order to explore the effect of low temperature stress on expression of MT, AQP1, and TCP1 genes in low temperature-resistant and non-low temperature-resistant strains of large yellow croaker. It was found that the expression of MT, AQP1 and TCP1 genes were affected by low temperature, with different expression levels between temperature-resistant strains and non-low temperature-resistant strains of large yellow croaker. The expression levels of MT and TCP1 genes in liver and muscle of low temperature-resistant strains and non-low temperature resistant strains were shown to be an upward trend as the decrease in water temperature, with peak expression level at 7 ℃. There was significantly higher expression level of MT gene in non-low temperature-resistant strains group than that in low temperature-resistant strains group(P<0.05). While there was significantly higher expression level of TCP1 gene in low temperature-resistant strains group than that in non-low temperature-resistant strains group (P<0.05). The expression levels of AQP1 gene in liver and muscle of large yellow croaker in each group were increased first and then decreased with the decrease in water temperature, with the peak expression level at 13 ℃, and the minimum at 7 ℃. The expression level of AQP1 was significantly lower in liver of low temperature-resistant strains than that in the non-low temperature resistant strains (P<0.05), without significant difference in the expression of AQP1 in muscle (P>0.05). The findings indicated that MT, AQP1, and TCP1 played an important role in the low temperature adaptation process of large yellow croaker, and were potential candidate genes for the study of cold tolerance mechanism of large yellow croaker. In addition, the different expression of the above three genes between low temperature-resistant strains and non-low temperature-resistant strains under low-temperature conditions also reflected the obvious different cold-resistant ability of low temperature-resistant strains and non-low temperature-resistant strains of large yellow croaker.
李伟业, 罗海忠, 殷小龙, 张川, 柳敏海, 章霞, 邱豪军, 毛志增, 油九菊, 徐志进. 低温胁迫对耐低温和非耐低温品系大黄鱼MT、AQP1、TCP1基因表达的影响[J]. 大连海洋大学学报, 2021, 36(1): 38-43.
LI Weiye, LUO Haizhong, YIN Xiaolong, ZHANG Chuan, LIU Minhai, ZHANG Xia, QIU Haojun, MAO Zhizeng, YOU Jiuju, XU Zhijin. Effects of low temperature stress on expression of MT, AQP1, and TCP1 genes in low temperature-resistant and non-low temperature- resistant strains of large yellow croaker Larimichthys crocea. Journal of Dalian Ocean University, 2021, 36(1): 38-43.
2021, Vol. 36 
