Expression analysis of RPL11/MDM2/P53 pathway related gene and protein in gills of fish exposed to cold stress
LIU Mingli1，2，3, YANG Wenyi1，2，3, WANG Jinfeng1，2，3, CHEN Liangbiao1，2，3*
1. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China;2. International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai 201306, China;3. National Demonstration Center for Experimental Fisheries Science Education, Shanghai 201306, China
Abstract: The differential expressions of several factors(related gene and protein)involved in RPL11/MDM2/P53 signaling pathway were investigated in gills of 6 month old Nile tilapia Oreochromisniloticus and zebrafish Daniorerio exposed to 8 ℃ from 28 ℃ in culture to evaluate the role of RPL11/MDM2/P53 under cold stress in cold tolerance in the two species fishes. Results showed that RPL11 and P53 protein expression was elevated in gill of tilapia with increase in cold stress period, whereas the zebrafish began to accumulate P53 in gills later than the tilapia did. The mdm2 mRNA expression level in tilapia gill was significantly increased after 6 h at 8 ℃(P<0.05), which may result in P53 activation. Expression level of P53 downstream target gene (bad and p21) was shown to be increased at 8 ℃ in 6 h after tilapia, with change in accordance with the change in P53 expression level. However, bad and p21 expression level of tilapia was different from zebrafish. The findings indicated that cold stress induced protein involved in apoptosis and related to RPL11/MDM2/P53 pathway expression change in tilapia, which was different from zebrafish which had stronger cold tolerance than tilapia. The species specific gene expression pattern may be involved in different cold tolerance abilities between tilapia and zebrafish.
刘明丽, 杨文意, 王金凤, 陈良标, . 低温胁迫下鱼类鳃中RPL11/MDM2/P53信号通路相关基因及蛋白表达差异分析[J]. 大连海洋大学学报, 2021, 36(1): 51-56.
LIU Mingli, YANG Wenyi, WANG Jinfeng, CHEN Liangbiao, . Expression analysis of RPL11/MDM2/P53 pathway related gene and protein in gills of fish exposed to cold stress. Journal of Dalian Ocean University, 2021, 36(1): 51-56.
2021, Vol. 36 
