Diversity of base sequences of mitochondrial ND4gene in six populations of Bagarius yarrelli
DU Min1, LI Na2, NIU Baozhen1, ZHOU Ya2, CHEN Chunli2, WEN Tianxian2, WANG Xinglong2
1. College of Life and Environmental Sciences, Key Laboratory of Health Aquaculture and Product Processing in Dongting Lake Area of Hunan Province, Hunan Engineering Research Center of Aquatic Organism Resources and Environmental Ecology, Zoology Key Laboratory of Hunan Higher Education, Hunan University of Arts and Science; 2.Key Laboratory for Quality, Efficient Cultivation and Security Control of Crops in Colleges and University of Yunnan Province, Honghe University
Abstract: A total of 72 mtDNA sequences of ND4 genes were analyzed in 12 samples of native fish Bagarius yarrelli collected from Manhao (Baya-M), the lower reaches of Nujiang River (Baya-N), Nujiangba-the upstream of Nujiang River(Baya-B), the upstream of Lanchangjiang(Baya-L), Jinhong-the lower reaches of Lanchangjiang(Baya-J), and Hekou-Honghe River(Baya-H). The results showed that the fish had ND4 gene with whole length of 1381 bp, 501 nucleotide mutation loci in 1381 loci and 67 haplotypes in 72 sequences. The nucleotide diversity was found to be changed from 0.001 9 to 0.072 2 and the relative genetic distance of 0.098 among 67 haplotypes. UPGMA method with Kimura 2-parameter model in MEGA software was used to construct phylogenetic tree among ND4 genes studied in this study and other two fish, Glyptothorax trilineatus and Gagata dolichonema were applied as outgroup, which denoted that B.yarrelli gathered to a branch. The findings indicated that there was relative small degree of differentiation between Hekou-Honghe River(Baya-H) and Manhao (Baya-M), showing relatively close genetic relationship, and that relatively large genetic distance between Lancanjiang(Baya-L) and Jinghong(Baya-J) (0.156), showing relatively far genetic relationship.
杜民, 李娜, 牛宝珍, 周亚, 陈春丽, 文天仙, 王兴龙. 6个巨魾群体线粒体ND4基因碱基序列多样性分析[J]. 大连海洋大学学报, 2020, 35(1): 103-107.
DU Min, LI Na, NIU Baozhen, ZHOU Ya, CHEN Chunli, WEN Tianxian, WANG Xinglong. Diversity of base sequences of mitochondrial ND4gene in six populations of Bagarius yarrelli. Journal of Dalian Ocean University, 2020, 35(1): 103-107.