Genetic diversity in two wild populations of tapertail anchovy Coilia mystus via 12S ribosomal segment sequences of mitochondrial DNA
CHENG Qi-qun, MA Chun-yan, MIAO Jiong, LU Xing,SHA Zhen-xia
1. Key Laboratory of Marine and Estuarine Fisheries, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China; 2. Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China; 3. School of Life Science, East China Normal University, Shanghai 200062, China; 4. Key Laboratory of Sustainable Utilization of Marine Fishery Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
Abstract: Genetic diversities in two populations of tapertail anchovy Coilia mystus, Yangtze River population and Pearl River population, were investigated by 12S ribosomal segment sequences of mitochondrial DNA. A total of forty- two individuals including twenty in Yangtze River populations and twenty -two in Pearl River population were collected. Sequences of 12S ribosomal （12SrRNA） amplified by PCR was used and 365 base pairs concensus sequences were obtained. Six different haplotypes were detected among all the 42 sequences, two in Yangtze River population and four in Pearl River population. The haplotype diversity index of Yangtze River population and Pearl River population was 0. 5263 and 0.6104, respectively. The nucleotide diversity of Yangtze River population and Pearl River population was 0. 144% and 0. 192%, respectively. Genetic distances were found to be 0.3% in the Yangtze River population, and between 0.3% and 0.5% in the Pearl River population. The genetic distances varied from 0.8% to 1.4% between the Yangtze River population and the Pearl River population. The Neighbour - Joining tree supported the two populations forming monophyletic group respectively. Analysis of molecular variance （AMOVA） analysis revealed that the variation between the populations accounted for 82.80% in total variation, suggesting that their genetic structure be isolated each other.
2007, Vol. 22 
