The tissue cultivation of fins and preparation of chromosomes in yellow catfish Pelteobagrus fulvidraco
SONG Li-min,WANG Wei-min,WANG Yu-fen
1.College of Fisheries Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction of Ministry of Education,Huazhong Agricultural University,Wuhan 430070,China;2.Beidaihe Central Experiment Station,Chinese Academy of Fishery Sciences,Qinhuangdao 066100,China
Abstract: A short-term tissue culture of fins in juvenile catfish Pelteobagrus fulvidraco was carried out by explant technique.Conditions of cell cultivation including disinfection method,culture medium,culturing temperature,content of colchicine and permeating time and chromosome preparation were studied to provide for easy operation,fast proliferation,and high-quality materials with related researches in a certain period of time.A set of fairly systemic experimental method to manufacture chromosomes of yellow catfish was established by fin tissue culture.
宋立民, 王卫民, 王玉芬. 黄颡鱼鳍组织培养及染色体制备条件的研究[J]. 大连海洋大学学报, 2010, 25(6): 502-505.
SONG Li-min, WANG Wei-min, WANG Yu-fen. The tissue cultivation of fins and preparation of chromosomes in yellow catfish Pelteobagrus fulvidraco. Journal of Dalian Ocean University, 2010, 25(6): 502-505.