Abstract: In the present study, the partial cDNA of CYP4 was amplified from the total RNA of epidermis muscle of the polychaete,Perinereis aibuhitensis, by reverse transcription with the primers based upon the CYP342A1 from Nereis virens. Then other primers were designed according to the partial gene of Perinereis aibuhitensis CYP4, and two fragments about 371 bp and 1044 bp were obtained by using 3＇ RACE and 5＇ RACE. Sequence analysis revealed a 1857 bp cDNA containing the 186 bp 5＇-untranslated region,225 bp 3＇ -untranslated regions and 1 446 bp open reading frame encoding 481 amino acid. The identity rate of deduced amino acid sequence of CYP4 was subjected to Blast searching in NCBI. The results showed that the CYP4 shared 73% identity with CYP4BB1,36% identity with CYP342A1 of Nereis virens and 39% identity with CYP4AT1 of Capitella capitate. The deduced amino acid sequence of CYP4 contained the conserved motifs of P450 family（FxxGxxxCxG） and had K-helix motifs of P450 family （ExxR）. In addition, it has the characteristic sequences of CYP4 family （EVDTFMFEGHDTT）. So it is in- ferred that the eDNA sequence might belong to the subfamily of CYP4B.
周一兵, 陈雪, 杨大佐, 万良, 王斌, 王丽丽, 孙静波. 双齿围沙蚕CYP4基因的克隆及序列分析[J]. 大连海洋大学学报, 2011, 26(6): 507-513.
ZHOU Yi-bing, CHEN Xue , YANG Da-zuo , WAN Liang , WANG Bin, WANG LI-li, SUN Jing-bo. Cloning and sequence analysis of cytochrome CYP4 family in polychaete Perinereis aibuhitensis. Journal of Dalian Ocean University, 2011, 26(6): 507-513.
2011, Vol. 26 
