The bacterial diversity in boifilms associated with media of anammox bioreactors
WANG Yin-hua, MA Yue-xin, LIU Chang-fa , ZHU Xue-hui, ZHU Ying
Key Laboratory of North Mariculture, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China; 2. College of Marine Science and Environment, Dalian Ocean University, Dalian 116023, China
Abstract: The bacterial diversity was studied in boifilms associated with media of two anammox bioreactors running at different time by PCR-DGGE using bacterial 16S rRNA universal primers 1055F/1392R-GC. Results showed that the bacterial diversity was different in two reactors at different sampling time. Phylogenetic analysis of sequences from prevalent DGGE bands revealed that the bacteria were found to be Thauera, Sphingomonas, Ectothiorhodospiraceae, Chloroflexi, Acidobacteria and uncultured bacteria. When the bioreactors were operated for 208 days, the removal rates of ammonia and nitrite nitrogens were kept high with the ratio of ammonia nitrogen level to nitrite nitrogen level = 1.1, indicating that the anammox reaction took place in the bioreactors. Anammox bacteria in the biofilms associated with media of bioreactors were detected by PCR-DGGE using anammox bacteria specific primers PIa46F/Amx368R-GC. Analysis of sequences from prevalent DGGE bands showed anammox bacteria had 96% similarity to Planctomycete KSU-1 and Candidatus Jettenia asiatica, respectively. It is suggested that these bacteria remove nitrogen by anaerobic ammonium-oxidation in reactors.
王银华, 马悦欣, 刘长发, 朱学惠, 朱莹. 厌氧氨氧化反应器载体生物膜细菌多样性的研究[J]. 大连海洋大学学报, 2011, 26(6): 500-506.
WANG Yin-hua, MA Yue-xin, LIU Chang-fa , ZHU Xue-hui, ZHU Ying. The bacterial diversity in boifilms associated with media of anammox bioreactors. Journal of Dalian Ocean University, 2011, 26(6): 500-506.
2011, Vol. 26 
