Abstract: A strain isolated from the diseased turbot Scophthalmus maximus was proved to be the pathogenic bacteria by an artificial infection test. The strain was identified as Edwardsiella tarda numbered as L-49231 strain. The adhesion and invasion of strain L-49231 to HaCaT cell was observed by being inoculated in 24-well cell culture plates. The adhesion and invasion of the L-49231 strain contacted to HaCaT cell during different period and transacted with different factors were studied. The results showed that the L-49231 was found to attach to the HaCaT cell and to invade into 2 hours after administration. The quantity of bacteria adhered and invaded to the cell increased with the increase in action period. The adhesive and invasion capability of L-49231 strain declined after treatment with Formaldehyde, Glutaraldehyde, Hydrochloric acid, and Trypsase. The adhesive capability varied little in disposal of Sucrose and D-mannose, but increased with Glucose. The results on invasion capability indicated that the invasion to the cell enhanced when the strain L-49231 was exposed to Glucose and D-mannose. The invasion of the L-49231 was exposed to Sucrose was partially restrained,but both its adhesion and invasion ability declined distinctly when the L-49231 was exposed to the antibody.
2010, Vol. 25 
