Effects of cryopreservation on antioxidant activities of semen enzymes in Russian sturgeon Acipenser gueldenstaedti
ZHANG Long-zhen, JIANG Qi, ZHUANG Ping, HUANG Xiao-rong, YAN Wen-gang
1. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key and Open Laboratory of Marine and Estuarine Fisheries Re- sources and Ecology, Ministry of Agriculture, Shanghai 200090, China ;2. College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, China; 3. Aquaculture Division, E-Institute of Shanghai Universites, Shanghai 201306, China
Abstract: The effects of cryopreservation（ -196 ℃ ）on the antioxidant activities of enzymes （SOD, CAT, GSH - PX and GR） in seminal plasma and spermatozoa of Russian sturgeon Acipenser gueldenstaedti were studied by a reagent kit to clarify damage mechanism of spermatozoa. The spermatozoa were found to show lower motility after preserved compared to the fresh semen, and the activities of SOD, CAT, GSH - PX in seminal plasma supplemented with cryoprotectants increased significantly （ P 〈 0.05 ） compared to the control group （ P 〈 0.05 ）. The activity of GR showed significantly lower compared to the fresh seminal plasma（ P 〈 0.05）, but significantly higher compared to the seminal plasma without supplementation of the cryoprotectants （ P 〈 0.05 ）. Compared to the fresh sperm, the spermatozoa supplementation of the cryoprotectants had lower activities of SOD, CAT, GSH - PX （P 〈 0.05）. However, the significantly higher activity of GR was observed in the spermatozoa supplementation of the cryoprotectants compared to the fresh sperm（P 〈0.05） ,and the significantly lower activity of GR was found in the seminal plasma without supplementation of the cryoprotectants （ P 〈 0.05 ）. The results showed that cryopreservation had significant effects on anti - oxidative system in the spermatozoa of Russian sturgeon and the motility of the spermatozoa.
2009, Vol. 24 
