Comparison of hypoxia stress tolerance among Manila clam Ruditapes philippinarum with four shell－colors
WANG Hua－min1， DING Jian－feng1，2， YANG Dong－min1， MU Zheng－qiang1，HUO Zhong－ming1，2， YAN Xi－wu1，2， BAO Lin1
1.College of Fisheries and Life Science， Dalian Ocean University， Dalian 116023， China； 2.Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province， Dalian 116023， China
Abstract: In this study， the expression of antioxidant enzymes(TPx， CAT， Mn－SOD， and Cu/Zn－SOD)and heat shock proteins(HSP40， HSP75， VpsHSP－1， and VpsHSP－2)family members were compared in Manila clam Ruditapes philippinarum with four shell－colors， as one intertidal shellfish in drastic change in dissolved oxygen level，exposed to 1 mg/L dissolved oxygen level to probe into the important role of antioxidant enzymes and heat shock proteins in the hypoxia stress in Manila clam.The results showed that first increase and then recovery to normal level in expression levels of members in the antioxidant gene family were found in mantle tissue in the four shell－color clam，the peak expression in 6 h hypoxia stress(P＜0.05).The expression of TPx gene in mantle tissue was significantly higher in white clam and CAT gene in the white zebra－colored clam than those in the clam with other three shell colors challenged with low dissolved O2(P＜0.05).The expression levels of genes in heat shock protein family in the gills were found to be first decrease and then to be increase in the white zebra－colored clam and zebra－colored clam，with the minimum at 6 h after the hypoxic stress(P＜0.05).The expression of these genes in wild clam and white clam was increased first and then reduced，with the maximal level in 6 h hypoxia stress in white color group(P＜0.05)， and in 12 h hypoxia stress in the wild clam(P＜0.05).The expression of heat shock proteins in mantle was reduced first and then increased in the white－zebra group， with the minimal level in 6 h hypoxia stress(P＜0.05)， while in the clam with other three shell colors the expression of heat shock proteins in mantle was increased first and then reduced， with the maximal level in 6 h hypoxia stress in wild clam and white shell－colored clam(P＜0.05)， and the maximal levels in zebra－colored clam in 12 h hypoxia stress(P＜0.05)，and significantly higher in white clam than those in the clam with other three shell colors(P＜0.05).The findings indicate that the TPx gene of zebra－colored clam and white clam may be response for process of the melanin synthesis， and that the genes related to melanin synthesis play an important role in hypoxia stress tolerance.
王化敏, 丁鉴锋, 杨东敏, 牟政强, 霍忠明, 闫喜武, 包琳. 4种壳色菲律宾蛤仔在低氧胁迫下的耐受能力比较研究[J]. 大连海洋大学学报, 2018, 33(2): 181-189.
WANG Hua－min, DING Jian－feng, YANG Dong－min, MU Zheng－qiang, HUO Zhong－ming, YAN Xi－wu, BAO Lin. Comparison of hypoxia stress tolerance among Manila clam Ruditapes philippinarum with four shell－colors. Journal of Dalian Ocean University, 2018, 33(2): 181-189.
2018, Vol. 33 
