Abstract: Juvenile Manila clam Ruditapes philippinarum from self inbred family line AA(♀A×♂A)and BB(♀B×♂B)and cross family line AB(♀A×♂B)and BA(♀B×♂A)was reared in a 500 L recirculating tank made of glass fiber reinforced plastics,indoor buckets and earthen ponds at water temperature of 20,24℃ and 28℃ and a salinity of 20,25 and 30 for 30-45 days to evaluate interactions between genotype and environment.Results showed that the temperature had a significant effect on the genotypes in genotype×temperature interaction effects, the order of shell length growth as BB>BA>AB>AA at 20 ℃, BA>AB>AA>BB at 24 ℃, and AA>BA>AB>BB at 28℃,without significant difference in the interaction between temperature and genotype(P>0.05).Salinity had a significant effect on genotypes in genotype× salinity interaction effects,significantly better growth in AA than that in BA,AB and BB(P<0.05),with the order of shell length growth as AA>AB>BA>BB,and without significant difference in the interaction between salinity and genotype(P>0.05).The feeding trials revealed that shell length growth in each family was significantly influenced by environmental factors, as outdoor earthen pond >circulating tank> water bucket.There was significant effects of environmental factors on genotype, significantly better growth in BA,AA and AB than that in BB(P<0.05),with AA>AB>BA>BB, and significant difference in the interaction between culture environment and genotype(P<0.05).The linear regression analysis of the interaction between four genotypes and culture environment indicated that the sensitivity order of each shell length growth genotype to different environments was expressed as BB>AB>BA>AA.The findings provide a scientific basis for the genetic breeding of Manila clam.