Cultivable microbiota in the gut of juvenile grouper Epinephelus coioides and in aquaculture water
LING Ze-ehun, YANG Hong-ling , SUN Yun-zhang, YE Ji-dan, LI Fu-dong, MA Ru-long
1. The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei Univ. , Xiamen 361021, China; 2. Xiamen Key Laboratory of Feed Detection and Safety Evaluation, Fisheries College, Jimei Univ. , Xiamen 361021, China; 3. Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural Univ. , Nanjing 210095, China
Abstract: The microbiota in the gut of juvenile grouper Epinephelus coioides and aquaculture water were isolated and purified by marine agar, TCBS and MRS media. The traits of the cultivable bacteria were analysed by physiological and biochemical tests in combination with 16S rRNA gene sequence analysis. The results showed that there were higher total cultivable bacterial number（9.0 × 106 cfu/g）and species in gut of the grouper than those in the aquaculture water（5.4 ×105 cfu/mL）. Seven bacterial species, however, were found in both the gut and aquaculture water, i.e. Vibrio parahaemolyticus, Vibrio harveyi, Bacillus pumilus, Bacillus clausii, Acinetobacter baumannii,Psychroacter sp. and Burkholderia cepacia, which were classified into 3 groups γ- proteobacteria, β - pro- teobacteria and Bacilli. Bacillus and Psychrobacter invading into the gut via the aquaculture water were found to be the predominant bacteria in the gut and aquaculture water. Three species of lactic acid bacteria, Lactococcus lactis, Lactobacillus casei and Enterococcusfaecium were isolated only from the gut, but comprising only 0.70% of the total bacteria. Furthermore, the amount of Vibrio in the gut and aquaculture water was very low, accounting for only 1.07% and 0.10% of the total bacteria, respectively.
凌泽春, 杨红玲, 孙云章, 叶继丹, 李富东, 马如龙. 斜带石斑鱼幼鱼消化道与养殖水体中可培养菌群的研究[J]. 大连海洋大学学报, 2009, 24(6): 497-503.
LING Ze-ehun, YANG Hong-ling , SUN Yun-zhang, YE Ji-dan, LI Fu-dong, MA Ru-long. Cultivable microbiota in the gut of juvenile grouper Epinephelus coioides and in aquaculture water. Journal of Dalian Ocean University, 2009, 24(6): 497-503.
2009, Vol. 24 
