Ultrastructural pathology of adhesion dysfunction disease in cultured juvenile sea cucumber Apostichopus japonicus
SONG Jian, WANG Pin-hong , LI Chun-yan , YANG Er-xin , CHANG Ya-qing
1. Key Laboratory of Mariculture ＆ Biotechnology, Agriculture Ministry, PRC, Dalian Fisheries Univ. , Dalian 116023, China;2. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;3. Graduate School of Chinese Academy of Sciences, Beijing 100039, China
Abstract: The epidemiology of sea cucumber Apostichopus japonicus was investigated from 2003 to 2005. In early summer of 2006, adhesion dysfunction disease （ADD） was broken out in the juvenile sea cucumber in many hatcheries in Dalian, Liaoning province, China. Electron microscopic observation on homogenate fluid revealed the presence of virus - like particles in the diseased juveniles. The virions are approximately 80 to 100 nm in diameter and have a smooth envelope without any spikes. Examination of ultrathin sections shows that the virions scattered in cytoplasm or congregated with encircled membrane. In cytopathic cells, endoplasmic reticulum and Golgi apparatus obviously swells and myline figures, the residues of lysosomes in cells appear as the feature of viral infection. No bacteria or other suspectable pathogens （rickettsia and chlamydia, bacteroidal, parasitic） were found.
2007, Vol. 22 
