Preliminary analysis of genetic variability and phylogenetic relationship among five species of Moina sp. （Cladocera）
ZHANG Peng , ZHAO Wen , LIU Wei-dong, YIN Xu-wang , SUN Jing-xian
1. Key Laboratory of Hydrobiology in Liaoning Province＇s University, Dalian Fisheries Univ. , Dalian 116023, China; 2. Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China
Abstract: The genetic variability and phylogenetic relationship among five species of Moina sp. were determined by RAPD technique. Under the optimal reaction conditions, twenty -one random primers had been used for amplification, in which 6 were found to produce reproducible and stable fragments. There were 161 polymorphic fragments The length of amplified fragment was ranged from 421 to 19329 bp. Based on the heredity distance, the program UPGMA and NJ of Mega software were applied for cluster analysis. The genetic diversity among different taxa and populations ranged from high to low as the following： M. affinis 〉 M. rectirostris 〉 M. macrocopa 〉 M. mongolica 〉 M. micrura. The RAPD markers could be helpful in the study of Moina＇s population differentiation and the identification of Moina.
2007, Vol. 22 
