Abstract: The chromosomal numbers and karyotypes of 5 species water fleas Moina were studied by the air - drying method. The chromosomes were classified by the methods of Levan et al. and Stebbins. The karyotype formula was found as following: 2N =24 =6M + 18T, NF =30, 1A in M. mongolica; 2N =22 = 10M + 12T, NF =32, 1A in M. macrocopa; 2N =24 = 12M + 12T, NF =36, 1B in M. rectirostris; 2N =24 = 10M + 14T, NF =34, 1A in M. micrura; 2N =26 = 12M + 14T, NF = 38, 1B in M. affinis. Furthermore, the evolutionary relationship among 5 species of Moina was discussed, and the comparable result revealed that the phylogenetic status was descendantly arranged as the following: M. rectirostris 〉 M. affinis 〉 M. macrocopa 〉 M. micrura 〉 M. mongolica.