The mannan- binding lectins from marine sponges Myxilla incrustans, Coelosphaera sp. and Biemna variantia
LI Wei1, Valentina Molchanova2, Irina Chikalovets2, Natalia Belogortseva2, Pavel Luk'yanov2
1.Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023,China; 2.Pacific Institute of Bioorganic Chemistry, Far East Branch of RAS, Vladivostok 690022, Russi
Abstract: We report the distribution of lectins from marine sponge Myxilla incrustans, Coelosphaera sp.,and Biemna variantia. In hemagglutination inhibition assays, mannan N1 was the most potent inhibitors for lectins from the three marine sponges. The binding site of three lectins from marine sponge recognizes the linkage α(1→ 2) and α(1→ 6) of the mannan from marine bacteria Bacillus pumilus. Among the glycoproteins tested in hemaggglutination inhibition assays, for the lectin from marine sponge Myxilla incrustans, Porcine Serum Mucin,Asialo-PSM,Fetuin and Asialo-fetuin were the best inhibitors. For the lectin from marine sponge Coelosphaera sp., PSM was the best inhibitors. A lectin from marine sponge Biemna variantia, PSM and Asialo-PSM were the best inhibitors. These lectins were found to agglutinate all types of human erythrocytes and the treatment of erythrocytes by tripsin. The hemagglutinating activity of these lectins was independent of the divalent cations Ca 2+.