Abstract: Morphology and cytochemistry of haemocytes in abalone, Haliotis discus hannai Ino, were described and their role in phagocytosis and encapsulation and mechanism of reactive oxygen species (ROS) production were studied. The hemocytes of abalone can be divided into two types: hyalinocytes(HY) and granulocytes (GR) based on the presence of granulates. There are granular particulates in HY with a basophilic cytoplasm, whereas GR contained eosinophilic granules. The HY comprised approximately 60% of the hemocytes in the abalone circulation. Both of the haemocytes, especially GR, were actively phagocytic. After stimulated with yeast, the blood cells were able to produce ROS which reduced NBT to formazan, accompanied by chemiluminescence (CL). There were less hemocytes and lower rate of the ROS production in starved abalones than those in the normal ones.
张剑诚, 张峰, 王吉桥. 皱纹盘鲍血细胞分类及活性氧产生机理的研究[J]. 大连海洋大学学报, 2004, 19(3): 182-188.
ZHANG Jian-cheng, ZHANG Feng, WANG Ji-qiao. Classification of hemocytes and mechanism of production of reactive oxygen species in abalone Haliotis discus hannai Ino. Journal of Dalian Ocean University, 2004, 19(3): 182-188.
2004, Vol. 19 
