Isolation, identification and anti-tumor activity of shell constituents in sea urchin Strongylocentrotus nudus
ZHU Chun-peng 1, YUAN Zhen-yu 1, SONG Yong-xiang 2, TONG Chang-qing 1, QU Min 1, JIN Qiao 1, LI Wei 1
1.College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China; 2.Key Laboratory of Marine Bio-resources Sustainable Utilization, Guangdong Key Laboratory of Marine Material Medicine, Research Center for Marine Microbes, South China Sea Institute of Oceanology, Chinese Academy of Science，Guangzhou 510301, China
Abstract: Two compounds were isolated from shells of sea urchin Strongylocentrotus nudus by column chromatography on silica gel and thin layer chromatography and identified by spectroscopic analysis and physical-chemistry properties. The anti-tumor activity of the two compounds was evaluated by the MTT assay and SRB assay. The results showed that the two compounds were identified as palmitate(compound 1) with unsaturated double bonds and glyceroyl monopalmitate (compound 2). In vitro trials revealed that the glyceroyl monopalmitate exhibited the potent inhibitory activities against five cancer cells, indicating that this compound is considered as potential anticancer candidate for further study.
2015, Vol. 30 
