Abstract: The influence of Cu2+concentration(0, 0.1, 0.5 and 1.0 mg/L) on behavior, blood parameters and antioxidant enzyme activity was studied in hybrid strugeon(European huso sturgeonHusohusoLinnaeus ♀×sterletAcipenserruthenusLinnaeus ♂) with body weight of (803.1±35.5)g for 24 h, 48 h and 72 h in a laboratory. The results showed that respiratory frequency, tail beating frequencies, and levels of red blood cell (RBC) and hemoglobin were increased with increase in concentration of Cu2+, and then decreased with Cu2+exposure elapse. The number of white blood cell (WBC), and catalase (CAT) and superoxide dismutase (SOD) activities were found to be dose-dependent, first increase, and then decrease with increase in concentrations of Cu2+. In the same concentration of Cu2+, however, the number of WBC and activities of antioxidant enzymes were decreased with Cu2+exposure elapse. The findings indicate that low concentration of Cu2+leads to induction of activities of antioxidant enzymes and activation of immune cells while high level of Cu2+results in inhibition of immune cells and activities of antioxidant enzymes.